Serum Protein Electrophoresis Procedure

The Helena TITAN GEL Serum Protein System is intend-ed for the separation and quantitation of serum proteins by agarose gel electrophoresis.

(prHWY.com) March 6, 2013 - TX, TX -- Texas, Mar 06, 2013 - The Helena TITAN GEL Serum Protein System is intend-ed for the separation and quantitation of serum proteins by agarose gel electrophoresis. Serum contains over one hundred individual proteins, each with a specific set of functions and subject to spe-cific variation in concentration under different pathologic conditions.1 Since the introduction of moving-boundary electrophore-sis by Tiselius2 and the subsequent use of zone elec-trophoresis, serum proteins have been fractionated on the basis of their electrical charge at a particular pH into five classical fractions: albumin, alpha 1 , alpha 2 , beta and gamma proteins. Each of these classical electrophoretic zones normally contains two or more components. Approximately fifteen serum proteins have been studied extensively because they may be measured easily. 3-5


Proteins are large molecules composed of covalently linked amino acids. Depending on electron distributions resulting from covalent or ionic bonding or structural sub-
groups, proteins can be either polar or nonpolar at a given pH. In the TITAN GEL Serum Protein procedure, proteins are separated according to their respective elec-
trical charges at 8.4-8.8 on agarose gel using both the electrophoretic and electroendosmotic forces present in the system. The proteins are then stained with Amido Black staining solution.


While some laboratorians prefer classic serum protein separations, others prefer a split-beta separation. With SPIFE Split-Beta gels, the proteins migrating in the beta region are split into two fractions, Beta 1 and Beta 2, between transferrin and C3 complement. In some instances, this may allow easier detection of small beta- migrating monoclonal gammopathies. Both the classic and split-beta formulations provide crisp, clear separation of the protein fractions.


Serum Protein Test - SPIFE 3000 is the top choice for clinical electrophoresis. Separations are superior and we continually look for ways to increase efficiency and labor savings. New urine protein and immunofixation assays that require no sample concentration are just one example.

The SPIFE 3000 separates and develops up to 1400 SPE samples or 210 IFE profiles in an 8-hour shift, with integrated sample and reagent application. Its barcode "lockout" feature provides positive ID between sample tray and electrophoresis gel.

About the Author:

Serum Protein - Helena Laboratories is a clinical laboratory instrument and reagent manufacturer. Our clients include major medical centers, small hospitals, large reference laboratories and small private doctor's laboratories.

Contact Details:

HELENA Laboratories
Beaumont, Texas,
USA

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