Bacterial Testing And Analysis - Sampling Procedures

Sampling Procedure for all Sewage Contamination Tests

Chatsworth, CA (prHWY.com) March 13, 2012 - 1. Obtain a sterile 1 mL Butterfield's Solution swab (EMSL Product ID 8708935) to collect and transport samples (provided at your request by EMSL).

2. With gloves on, remove swab from sterile packaging.

3. Carefully unscrew cap of sampling device - swab is attached to the lid of the cap.

4. Gently press out excess solution from sampling swab by pressing the swab against the inside wall of the tube with a rolling motion.

5. Hold swab at an approximate 30° angle from the sampling surface, taking care not to contaminate any part of the swab or the sampling site.

6. Using firm, even pressure move the swab slowly and thoroughly over an entire 4" x 4" sampling area, rewetting the swab tip with the Neutralizing Solution as needed. First horizontally, then vertically.

7. After sampling is complete, carefully put swab back into vial and close cap tight.

8. Label the sample using a permanent ink marker.

9. Background samples in non-fecally contaminated areas should be taken.

Sample Shipping

· Samples that will be submitted for culture-based analysis must be shipped overnight cold, not frozen, within 24 hours from the sample collection. The best method of shipment for these samples is to use a cooler with freezer packs, not bags of ice or loose ice.

· There are no special shipping requirements or hold times for Bacteroides since they will not multiply outside of the intestine. However, do not store sample longer than a month before analysis.


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